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|Title:||Inactivation of IL-15 gene expression does not reduce atherosclerosis in a mouse model of carotid artery narrowing|
|Department:||Biochemistry and Biomedical Sciences|
|Abstract:||IL-15 is a pleiotropic cytokine which influences a variety of immune and inflammatory responses. Our lab has previously demonstrated that IL-15 and IL-15Rα promote atherosclerosis. However, the mechanisms by which IL-15 affect atherosclerosis development were not fully defined. In this study, we reported that overexpression of the IL-15 gene resulted in an increase of granzyme B level in the atherosclerotic plaque of ApoE deficient mice. Furthermore, we observed that leukocytes-specific genetic deletion of IL-15Rα reduced the granzyme B level within atherosclerotic lesions from LDLr deficient mice. Collectively, our data shows one of the mechanistic pathways by which IL-15 promotes atherosclerosis development. Moreover, we tested the role of IL-15 in carotid artery disease. It has been reported previously that immunization of low density lipoprotein receptor deficient mice against IL-15, by inoculating them with bacteria harboring an IL-15 expression plasmid, led to reduced development of diet induced atherosclerosis in carotid arteries whose diameter was restricted to induce a hemodynamic stress. Others, however, reported that injection of wild type mice with an antibody against IL-15 triggered increased neointima formation in carotid arteries that were partially ligated. In our study, we found no differences in the amount of collar/diet induced atherosclerosis in control apoE KO mice and in IL-15/apoE dKO mice.Therefore,inactivation of IL-15 gene expression does not appear to affect the rapid onset of atherosclerosis in carotid arteries of ApoE KO mice induced by a combination of high fat diet and hemodynamic stress.|
|Appears in Collections:||Open Access Dissertations and Theses|
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|Alshuweishi_Yazeed_201604_Master.pdf||2.11 MB||Adobe PDF||View/Open|
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