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http://hdl.handle.net/11375/18265
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DC Field | Value | Language |
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dc.contributor.advisor | Jacobs, Roger | - |
dc.contributor.author | Raza, Qanber | - |
dc.date.accessioned | 2015-09-25T19:46:41Z | - |
dc.date.available | 2015-09-25T19:46:41Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.uri | http://hdl.handle.net/11375/18265 | - |
dc.description.abstract | Collective cell migration is a defining feature of many morphogenetic processes. Diseases such as congenital heart diseases and cancer arise due to mis-regulation of collective migratory behaviour and animal models have played a pivotal role in dissecting the molecular mechanisms which underlie this process. During embryonic heart development, cardiac precursors undergo a stage of collective migration in both vertebrates and invertebrates. We developed a paradigm to quantitatively assess collective cell migration of cardiac precursors in live embryos of Drosophila, which is the simplest genetic model organism with a heart. Therefore, we studied processes which are commonly observed in most collective cell migration models such as guidance signalling and extracellular matrix remodelling. Our results demonstrate that leading edge of migrating cardioblasts is highly active and that this behaviour is regulated by guidance cues, Slit and Netrin and their respective receptors Robo/Robo2 and Frazzled/Uncoordinated5. These molecules cooperatively promote leading edge motility and epithelial characteristics of the cardioblasts. Next, we determined that matrix restructuring around the cardioblasts requires proteases Mmp1 and Mmp2, which are members of the highly conserved Matrix Metalloproteinase family. We demonstrate that Mmp1 and Mmp2 have distinct roles during lumen formation, however, both Mmp1 and Mmp2 are required for collective motility of the cardioblast leading edge. Hence, we propose that embryonic heart development in Drosophila is an effective and amenable model of collective cell migration which can be applied to discover unique mechanisms which coordinate cell movement in groups. | en_US |
dc.language.iso | en | en_US |
dc.subject | Collective cell migration guidance signalling extracellular matrix metalloproteinases mmp slit robo netrin frazzled uncoordinated5 metastasis congenital heart disease heart development morphogenesis filopodia lamellopodia | en_US |
dc.title | COLLECTIVE CELL MIRATION DURING HEART MORPHOGENESIS IN DROSOPHILA REQUIRES GUIDANCE SIGNALING AND EXTRACELLULAR MATRIX REMODELLING | en_US |
dc.title.alternative | COLLECTIVE CELL MIGRATION OF CARDIOBLASTS DURING HEART MORPHOGENESIS | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biology | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Doctor of Philosophy (PhD) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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Raza_Qanber_S_2015_September_PhD.pdf | Doctor of Philosophy Thesis in Developmental Biology and Genetics | 5.92 MB | Adobe PDF | View/Open |
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