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|Title:||In situ gelling hydrogel for topical and mucosal applications|
|Abstract:||Hydrogel networks have attracted considerable research interest due to their potential in multiple biomedical applications, including drug delivery, wound healing, tissue engineering, and biomedical equipment coatings. In particular, multi-functional hydrogels that are injectable, non-cytotoxic, anti-bacterial, and bioadhesive offer potential applications in wound management, surgical barrier materials, and mucosal or skin-based drug delivery. In this thesis, an in situ injectable hydrogel network based on chitosan and poly(oligoethylene glycol methacrylate (POEGMA) was prepared using covalent aldehyde-hydrazide chemistry to form a stable hydrazone bond that is biodegradable, biocompatible, and adaptable to form networks with varying mechanical properties. Chitosan, a cationic polymer, has inherent adhesive and antibacterial properties, while POEGMA is innately protein-repellent and non cytotoxic. Therefore, by combining these materials, a non-cytotoxic and degradable hydrogel with both adhesive and antibacterial properties can be produced, with potential applications in drug delivery and wound healing. The focus of this thesis is to functionalize chitosan to possess hydrazide groups without losing its inherent adhesive and antibacterial characteristics. This was be confirmed through titration and FT-IR to confirm and quantify the degree hydrazide functionalization followed by typical hydrogel characterization such as gelation time, degradation kinetics at different pH, transparency, and mechanics to ensure the required criteria for potential use in vaginal and skin applications are all met. The adhesive properties of the hydrogel with biological tissue (porcine skin) were assessed using peel and tack testing strategies, while mucoadhesion potential was screened using tack tests relative to a model mucosal membrane. Antibacterial properties of the functionalized chitosan polymer and chitosan-POEGMA hydrogel were characterized using minimum inhibitory concentration measurements, zone of inhibition assays, macro broth dilution, and LIVE/DEAD bacterial assay using E.coli as the bacterial model. Different chitosan weight percentage incorporations in the hydrogel matrix were investigated and compared, both internally as well as with a cationic POEGMA-POEGMA hydrogel to identify the role of chitosan in controlling the gel properties.|
|Appears in Collections:||Open Access Dissertations and Theses|
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|Elmajdoub_Juhina_M_201509_MAScBiomedicalEngineering.docx||Thesis||4.32 MB||Microsoft Word XML||View/Open|
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