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http://hdl.handle.net/11375/17968| Title: | Temperature-Sensitive Mutants of Bacteriophage PBS 2 |
| Authors: | Herrington, Muriel Bella |
| Advisor: | Takahashi, I. |
| Department: | Biology |
| Keywords: | biology;temperature-sensitive mutant;bacteriophage; PBS 2 |
| Publication Date: | Nov-1973 |
| Abstract: | <p> Temperature-sensitive mutants of the bacteriophage PBS 2 were isolated from lysates treated with various mutagens. Complementation tests assigned the mutants to 10 cistrons. The mutants were mapped by two factor crosses and formed a linear map approximately 50 recombination percent in length. </p> <p> A method for phage transformation was developed. By the use of wild type DNA fragments fractionated according to their guanine + cytosine content in HgCs₂SO₄ gradients, it was possible to determine the base composition of certain regions of the chromosomes. </p> |
| URI: | http://hdl.handle.net/11375/17968 |
| Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Herrington_Muriel_B_1973Nov_PhD.pdf | 24.31 MB | Adobe PDF | View/Open |
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