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http://hdl.handle.net/11375/17765
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DC Field | Value | Language |
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dc.contributor.advisor | Neilson, Thomas | - |
dc.contributor.author | England, Thomas Edward | - |
dc.date.accessioned | 2015-07-16T22:06:54Z | - |
dc.date.available | 2015-07-16T22:06:54Z | - |
dc.date.issued | 1976-10 | - |
dc.identifier.uri | http://hdl.handle.net/11375/17765 | - |
dc.description.abstract | <p> An improved method for the synthesis of oligoribonucleotides of defined sequence was developed. The general phosphotriester synthesis of Neilson and co-workers was modified by the introduction of a new condensing agent, mesitylenesulfonyl-1,2,4-triazole, and by the replacement of the bis(cyclohexylammonium) salt of mono-2,2,2-trichloroethyl phosphate with its acid salt. These modifications provided significant increases in the yields for the condensation of protected nucleosides - especially in the case of purine residues. Finally, modification of the three-step procedure for the deprotection of protected oligoribonucleotides resulted in the isolation of oligomers of exceptional purity and biological activity.</p> <p> Oligomers corresponding to natural sequences in transfer RNA molecules were obtained by this improved method of synthesis. These oligomers were then used to study: 1. The formation of short double-stranded RNA helices and 2. The interactions of aminoacyl-tRNA ligases with tRNA fragments.</p> | en_US |
dc.language.iso | en_US | en_US |
dc.subject | oligoribonucleotides, salt, RNA, molecules, Double-stranded, synthesis, hybridization | en_US |
dc.title | Synthesis and Hybridization Studies of Oligoribonucleotides Corresponding to the Common, Double-Stranded Region of the Dihydrouridine Arm of Several Transfer RNA Molecules | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Biochemistry | en_US |
dc.description.degreetype | Thesis | en_US |
dc.description.degree | Doctor of Philosophy (PhD) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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England_Thomas_E._1976Oct_Ph.D..pdf | 6.15 MB | Adobe PDF | View/Open |
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