Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/17765
Title: | Synthesis and Hybridization Studies of Oligoribonucleotides Corresponding to the Common, Double-Stranded Region of the Dihydrouridine Arm of Several Transfer RNA Molecules |
Authors: | England, Thomas Edward |
Advisor: | Neilson, Thomas |
Department: | Biochemistry |
Keywords: | oligoribonucleotides, salt, RNA, molecules, Double-stranded, synthesis, hybridization |
Publication Date: | Oct-1976 |
Abstract: | <p> An improved method for the synthesis of oligoribonucleotides of defined sequence was developed. The general phosphotriester synthesis of Neilson and co-workers was modified by the introduction of a new condensing agent, mesitylenesulfonyl-1,2,4-triazole, and by the replacement of the bis(cyclohexylammonium) salt of mono-2,2,2-trichloroethyl phosphate with its acid salt. These modifications provided significant increases in the yields for the condensation of protected nucleosides - especially in the case of purine residues. Finally, modification of the three-step procedure for the deprotection of protected oligoribonucleotides resulted in the isolation of oligomers of exceptional purity and biological activity.</p> <p> Oligomers corresponding to natural sequences in transfer RNA molecules were obtained by this improved method of synthesis. These oligomers were then used to study: 1. The formation of short double-stranded RNA helices and 2. The interactions of aminoacyl-tRNA ligases with tRNA fragments.</p> |
URI: | http://hdl.handle.net/11375/17765 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
England_Thomas_E._1976Oct_Ph.D..pdf | 6.15 MB | Adobe PDF | View/Open |
Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.