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http://hdl.handle.net/11375/15357
Title: | Primary Cilia Dynamics, Morphology and Acetylation are Abnormal in Huntington’s Disease Cell Models |
Authors: | Woloshansky, Tanya S. |
Advisor: | Truant, Ray Guarne, Alba Burrows, Lori |
Department: | Biochemistry and Biomedical Sciences |
Keywords: | Primary Cilia;Huntington's disease;Huntingtin;Acetylation |
Publication Date: | Oct-2014 |
Abstract: | <p>The primary cilium is a singular signaling organelle found on most mammalian cell types. Dysfunction of the primary cilium or associated structures form a group of genetic disorders called ciliopathies. Recently, Huntington’s disease (HD), a monogenetic neurodegenerative disorder, was classified, at least in part, as a ciliopathy. How the primary cilium contributes to the pathogenesis of HD is the focus of this work. We demonstrate that huntingtin localization to the basal body or primary cilium is dependent on the phosphorylation status of serine residues 13 and 16. Furthermore, we demonstrate that, compared to controls, HD cell models have an increased number of cells with a primary cilium and that these cells have higher presence of huntingtin within the ciliary compartment. The primary cilia that form in HD cell lines demonstrate abnormal dynamics and morphology with bulging tips, characteristic of defective retrograde trafficking. We also demonstrate that alpha tubulin acetyltransferase 1 (αTAT1) expression and localization is increased in the primary cilium of HD cell lines. Subsequently, the primary cilium of HD cell lines are highly acetylated when compared to controls. These data support that primary cilia structure, ciliogenesis and ciliome are altered in HD.</p> |
URI: | http://hdl.handle.net/11375/15357 |
Identifier: | opendissertations/9078 10033 5537170 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
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fulltext.pdf | 2.03 MB | Adobe PDF | View/Open |
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