Skip navigation
  • Home
  • Browse
    • Communities
      & Collections
    • Browse Items by:
    • Publication Date
    • Author
    • Title
    • Subject
    • Department
  • Sign on to:
    • My MacSphere
    • Receive email
      updates
    • Edit Profile


McMaster University Home Page
  1. MacSphere
  2. Open Access Dissertations and Theses Community
  3. Open Access Dissertations and Theses
Please use this identifier to cite or link to this item: http://hdl.handle.net/11375/13526
Full metadata record
DC FieldValueLanguage
dc.contributor.advisorBoreham, Douglasen_US
dc.contributor.advisorAndrews, David W.en_US
dc.contributor.advisorParise, Giannien_US
dc.contributor.authorMills, Caitlin E.en_US
dc.date.accessioned2014-06-18T17:04:19Z-
dc.date.available2014-06-18T17:04:19Z-
dc.date.created2013-09-24en_US
dc.date.issued2013-10en_US
dc.identifier.otheropendissertations/8361en_US
dc.identifier.other9409en_US
dc.identifier.other4624163en_US
dc.identifier.urihttp://hdl.handle.net/11375/13526-
dc.description.abstract<p>Ionizing radiation is used to screen for, diagnose, and treat breast cancer. Noncancerous breast cells are exposed to a range of doses as a result of these procedures in addition to the low levels of natural background radiation that they are always exposed to. With the consistent increase in medical radiation procedures, and the climbing rates of breast cancer in most populations, understanding the effects of these exposures is of interest and importance. Radiation exposure results in DNA double strand breaks which can trigger various cellular responses including cell cycle arrest, and cell death. Apoptosis is a form of highly regulated cell death that is controlled by the Bcl-2 family of proteins. Bcl-2 and Bcl-XL are anti-apoptotic proteins that are commonly overexpressed in human cancers including breast cancer. This thesis was focused on investigating the effects of Bcl-2 and Bcl-XL expression on the radiation response of human mammary epithelial cells. Bcl-2 and Bcl-XL were exogenously expressed in MCF-10A cells, and Bcl-XL expression was knocked down. Cytogenetic techniques were used to characterize the MCF-10A cells used. Automated image based assays were used to validate the anti-apoptotic effects of the exogenous proteins against known apoptotic stimuli, and to probe the effects of radiation exposure. The influence of Bcl-2 and Bcl-XL expression on the immediate and short term effects of radiation exposure was investigated using cell growth assays, flow cytometry, and</p>en_US
dc.subjectRadiationen_US
dc.subjectBreast Canceren_US
dc.subjectApoptosisen_US
dc.subjectMammographyen_US
dc.subjectAutomationen_US
dc.subjectImagingen_US
dc.titleThe Effects of Bcl-2 and Bcl-XL Expression on the Radiation Response of MCF-10A Cellsen_US
dc.typethesisen_US
dc.contributor.departmentMedical Physicsen_US
dc.description.degreeDoctor of Philosophy (PhD)en_US
Appears in Collections:Open Access Dissertations and Theses

Files in This Item:
File SizeFormat 
fulltext.pdf
Open Access
5.43 MBAdobe PDFView/Open
Show simple item record Statistics


Items in MacSphere are protected by copyright, with all rights reserved, unless otherwise indicated.

Sherman Centre for Digital Scholarship     McMaster University Libraries
©2022 McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L8 | 905-525-9140 | Contact Us | Terms of Use & Privacy Policy | Feedback

Report Accessibility Issue