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http://hdl.handle.net/11375/12653
Title: | GLUCOSE SUPPLEMENTATION AND MEASURES OF OXIDATIVE STRESS IN PATIENTS WITH GLYCOGEN PHOSPHORYLASE DEFICIENCY (MCARDLE’S DISEASE). |
Authors: | Mocellin, Joseph Nicholas |
Advisor: | Tarnopolsky, Mark |
Department: | Medical Sciences |
Keywords: | McArdles disease;oxidative stress;exercise;glucose supplementation;Biochemical Phenomena, Metabolism, and Nutrition;Medical Nutrition;Biochemical Phenomena, Metabolism, and Nutrition |
Publication Date: | Oct-2012 |
Abstract: | <p><em>Objective: </em>We evaluated the potential effect of oral glucose supplementation on: (1) exercise performance and tolerance, (2) the concentrations of plasma uric acid and ammonia (NH<sub>3</sub>) and (3) blood plasma markers of oxidative stress in patients with McArdle’s disease (MCD) after non-ischemic forearm exercise testing (non-ischemic forearm exercise test). <em>Methods:</em> Blood samples and exercise performance measures were performed on from 16 patients with MCD and 17 control subjects (CON) matched for age, sex and physical activity status. Subjects performed 2 exercise bouts 30 minutes apart and received oral glucose or placebo supplementation between tests. Blood samples were analyzed for concentrations of 8-isoprostanes (8-ISO), malondialdehyde (MDA) and total anti-oxidant capacity (TEAC). Exercise performance was assessed using a handgrip dynamometer to measure force of contraction over time. Exercise tolerance was assessed based on subject’s self-reported perception of pain and perception of exertion during exercise. <em>Results:</em> MCD was associated with greater fatigue, perceived pain, perceived exertion, and higher uric acid during non-ischemic forearm exercise test (P < 0.05), and higher concentrations of plasma NH<sub>3 </sub>post exercise (P < 0.05). Glucose did not influence plasma uric acid or NH<sub>3 </sub>and had no effect on exercise measures in MCD patients. Baseline plasma markers of oxidative stress were not different between MCD patients and CON; however, MCD patients who ingested glucose between non-ischemic forearm exercise tests had lower plasma 8-ISO concentrations (P < 0.05). CON who ingested glucose between non-ischemic forearm exercise tests had lower plasma 8-ISO concentrations (P < 0.05) at +1 min post exercise compared to initial non-ischemic forearm exercise test. The TEAC of control subjects was lower following non-ischemic forearm exercise test (P < 0.05), with no change in MCD patients. <em>Conclusion: </em>Glucose may have a protective effect on oxidative stress following exercise that may be due to attenuated flux through xanthine oxidase.</p> |
URI: | http://hdl.handle.net/11375/12653 |
Identifier: | opendissertations/7520 8579 3352636 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
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fulltext.pdf | 3.04 MB | Adobe PDF | View/Open |
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