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http://hdl.handle.net/11375/12422
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DC Field | Value | Language |
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dc.contributor.advisor | Coombes, Brian K. | en_US |
dc.contributor.author | Osborne, Suzanne | en_US |
dc.date.accessioned | 2014-06-18T16:59:34Z | - |
dc.date.available | 2014-06-18T16:59:34Z | - |
dc.date.created | 2012-08-31 | en_US |
dc.date.issued | 2012-10 | en_US |
dc.identifier.other | opendissertations/7310 | en_US |
dc.identifier.other | 8358 | en_US |
dc.identifier.other | 3280943 | en_US |
dc.identifier.uri | http://hdl.handle.net/11375/12422 | - |
dc.description.abstract | <p>Originally considered the sole providence of protein coding sequences, evolutionary biology has begun to recognize the importance of non-coding DNA in dictating phenotypic adaptation. Exclusively examined in eukaryotic anatomical development, <em>cis</em>-regulatory modifications have the power to alter the spatial-temporal dynamics of gene expression without the plieotropic consequences of protein modification. Owing to the need to integrate horizontally acquired DNA into existing regulatory networks, <em>cis</em>-regulatory mutations may also significantly contribute to prokaryotic evolution. The horizontal acquisition of <em>Salmonella</em> Pathogenicity Island (SPI)-2 led to the evolutionary divergence of <em>Salmonella enterica</em> from <em>S. bongori</em>. Use of the type 3 secretion system encoded in SPI-2 allowed <em>S. enterica</em> to exploit an intracellular host niche offered by immune cells and allowed for its systemic dissemination. Here we identify ancestrally encoded <em>srfN</em> and <em>dalS</em> and demonstrate that through acquisition of a binding site for the SPI-2 regulator, SsrB, they have contributed to the pathoadaptation of <em>S. enterica</em> to the host environment. We also demonstrate that ancestral regulatory networks contribute to the establishment of an expression hierarchy for SPI-2 <em>in vitro</em> and to transcriptional priming in the host lumen prior to invasion. These findings demonstrate that <em>cis</em>-regulatory modifications have significantly contributed to the evolution of <em>S. enterica</em> as an intracellular pathogen.</p> | en_US |
dc.subject | Salmonella | en_US |
dc.subject | evolution | en_US |
dc.subject | infection | en_US |
dc.subject | Pathogenic Microbiology | en_US |
dc.subject | Pathogenic Microbiology | en_US |
dc.title | Cis-Regulatory Evolution in Salmonella enterica | en_US |
dc.type | dissertation | en_US |
dc.contributor.department | Biochemistry | en_US |
dc.description.degree | Doctor of Philosophy (PhD) | en_US |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Size | Format | |
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fulltext.pdf | 4.05 MB | Adobe PDF | View/Open |
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